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Associate Professor Chatchawan Srisawat

- M.D. (first class honor) Faculty of Medicine, Siriraj Hospital, Mahidol University
- Ph.D. (Biological Chemistry), University of Michigan (Ann Arbor)

Office
1016 Srisavarindira Bldg. 10th Fl. Department of Biochemistry.

Faculty of Medicine, Siriraj Hospital , Bangkoknoi, Bangkok 10700

Laboratory

916 Srisavarindira Bldg. 9rd Fl.

Phone: (02) 419-9144
E-mail: chatchawan.sri@mahidol.ac.th

 

Research Profile

In the early1990s, a new class of molecules called “aptamer” has been developed. Aptamers are single-stranded oligonucleotides (either DNA or RNA) that can bind to target molecules with affinity and specificity comparable to antibodies. Aptamers can be developed using in vitro selection or SELEX techniques (Systematic Evolution of Ligands by EXponential enrichment) as summarized in the diagram below. The techniques are very powerful in allowing the isolation of rare RNA or DNA molecules, or "aptamers", from a pool of random RNAs or DNAs of more than 1016 different sequences. The selection process starts with a library of RNAs with randomized sequences. The library is generated from ss-DNA containing a randomized region flanked by constant sequences at both 5' and 3' ends, which are used as primer binding sites during RT-PCR amplification. In addition, the promoter of T7 RNA polymerase is incorporated into the 5' end of ss-DNAs. This random ss-DNA molecules are converted into double-stranded DNAs (ds-DNA) by PCR, and the ds-DNAs are subsequently used as templates for in vitro transcription using T7 RNA polymerase to generate a pool of random RNAs. The complexity of the library depends on the number of nucleotides present in the randomized regions (varied from 25-80 nucleotides long). The commonly-used libraries have the complexity of ~ 10^15-10^16 different sequences. The aptamers are partitioned from the noninteracting RNA ligands based on their ability to bind the target ligands. The pool of RNAs recovered after partitioning is used to generate ds-DNA by RT-PCR amplification. The ds-DNA templates are then used for in vitro transcription to produce a new pool of RNA for use in the next round of selection. The selection process is repeated until the aptamers with desired properties are highly enriched. Because the random RNA library with a large complexity is used, it is likely that some of the molecules could fold into the conformations that are able to recognize and bind to the selected target molecules even though they are not natural ligands for RNAs. So far, aptamers to a wide range of targets have been discovered, ranging from simple ions, small molecules, peptides, single proteins, organelles, viruses and even entire cells.

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Since the invention of the aptamer technology, aptamers have now become an emerging class of molecules that can rival antibodies in both therapeutic and diagnostic applications. In addition to the comparable affinity and specificity of aptamers and antibodies to target molecules, there are several desirable properties of aptamers over those of antibodies. For example, the production of aptamers is faster and cheaper because the selection processes are done in vitro and no animals involved. Aptamers can also be raised against some antigenic epitopes on the target protein that are important for activity or functions but have poor immunogenicity because the selection of aptamers is not based on the immunological response. In addition, because aptamers are nucleic acids, which are not good immunogens and have a shorter half-life in the body, they could be used more safely as a therapeutic agent compared with antibodies, particular those produced from other species.

We are currently developing RNA aptamers to the dengue virus envelope protein with neutralizing activity against dengue virus infection. The aptamers might be useful for diagnostics or therapeutics of dengue infection. In addition, we are interested in generating RNA aptamers with specificity to various types of hemoglobin in order to use them for diagnosis of hemoglobinopathies or thalassemia, which is one of the most common hematologic diseases in Thailand. In the future, we plan to develop RNA aptamers against target molecules that could be used in diagnostics or therapeutics of the diseases with medical importance in Thailand.

Besides the aptamer project, we are also studying the suppression of collagen production in keloid fibroblasts using a pharmacologic agent, curcumin, and RNA interference against collagen genes, i.e. COL1A1 and COL1A2. The results from this study might be useful for treatment of keloids in the future.

 

Publications

1

Bachtiar BM, Srisawat C, Bachtiar EW. RNA aptamers selected against yeast cells inhibit Candida albicans biofilm formation in vitro. Microbiologyopen. 2019 Feb 18:e812. doi: 10.1002/mbo3.812.

2

Saisuk W, Srisawat C, Yoksan S, Dharakul T. Hybridization Cascade Plus Strand-Displacement Isothermal Amplification of RNA Target with Secondary Structure Motifs and Its Application for Detecting Dengue and Zika Viruses.  Anal Chem. 2019 Feb 11. doi: 10.1021/acs.analchem.8b03736.

3

Trakarnsanga K, Wilson MC, Heesom KJ, Andrienko TN, Srisawat C, Frayne J. Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro. Sci Rep. 2018 Jan 31;8(1):1983. doi: 10.1038/s41598-018-20491-1.

4

Kooptiwut S, Wanchai K, Semprasert N, Srisawat C, Yenchitsomanus PT. Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose. Sci Rep. 2017 Nov 30;7(1):16639. doi: 10.1038/s41598-017-15237-4.

5

Tummarintra P, Limratana P , Sujirattanawimol K , Srisawat C. The contamination of intravenous fluid by felt-tip marking pen ink: a pilot study. Siriraj Medical Journal.2017 Aug 20; 70(4):349-354. doi:10.14456/smj.2018.55

6

Thuangtong A, Lertpongparkpoom D, Srisawat C, Junnu S, Pratumvinit B. Deposition of Triglyceride on Soft Contact Lenses from Lipid-Containing Artificial Tears. Siriraj Med J. 2016;68:241-246

7

Leela SL, Srisawat C, Sreekanth GP, Noisakran S, Yenchitsomanus PT, Limjindaporn T.Drug repurposing of minocycline against dengue virus infection. Biochem Biophys Res Commun. 2016 Sep 9;478(1):410-6. doi: 10.1016/j.bbrc.2016.07.029.

8

Srisawat C, Nakponetong K, Benjasupattananun P, Suratannon C, Wachirutmanggur L, Boonchoo S, Pankaew D, Laocharoenkiat A, Pacharn P,Jirapongsananuruk O, Visitsunthorn N, Vichyanond P.A preliminary study of intranasal epinephrine administration as a potential route for anaphylaxis treatment. Asian Pac J Allergy Immunol. 2016 Mar;34(1):38-43.

9

Panya A, Sawasdee N, Junking M, Srisawat C, Choowongkomon K, Yenchitsomanus PT. A peptide inhibitor derived from the conserved ectodomain region of DENV membrane (M) protein with activity against Dengue virus infection. Chem Biol Drug Des. 2015 Apr 17. doi: 10.1111/cbdd.12576.

10

Sreekanth GP, Chuncharunee A, Sirimontaporn A, Panaampon J, Srisawat C, Morchang A, Malakar S, Thuwajit P, Kooptiwut S, Suttitheptumrong A, Songprakhon P, Noisakran S, Yenchitsomanus PT, Limjindaporn T.Role of ERK1/2 signaling in dengue virus-induced liver injury.Virus Res. 2014 Apr 1;188C:15-26. doi: 10.1016/j.virusres.2014.03.025. [Epub ahead of print]

11

Thuangtong R, Maneeprasopchoke P, Srisawat C, Vatanashevanopakorn C, Hanamornroongruang S, et al. In vitro Culture and Histological Characterization of Extracted Human Hair Follicles.J Clin Exp Dermatol Res 4:182. 2013 Sep 10. doi: 10.4172/2155-9554.1000182

12

Soi-ampornkul R, Srisawat C, Thangnipon W, Kanyok S, Junnu S, Katanyoo W, Liammongkolkul S, Soi-ampornkul P. Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal Sk-N-Sh Cells. International Journal of Nutrition and Food Sciences. Vol. 2, No. 4, 2013, pp. 167-173. doi: 10.11648/j.ijnfs.20130204.12

13

Nagila A, Netsawang J, Suttitheptumrong A, Morchang A, Khunchai S, Srisawat C, Puttikhunt C, Noisakran S, Yenchitsomanus PT, Limjindaporn T.

Inhibition of p38MAPK and CD137 signaling reduce dengue virus-induced TNF-alpha secretion and apoptosis.Virol J. 2013 Apr 4;10(1):105.

14

Srisawat C, Junnu S, Peerapittayamongkol C, Futrakul A, Soi-Ampornkul R, Senanarong V, Praditsuwan R, Assantachai P, Neungton N. The platelet amyloid precursor protein ratio as a diagnostic marker for Alzheimer's disease in Thai patients. J Clin Neurosci. 2013 Feb 26. doi:pii: S0967-5868(12)00506-1. 10.1016/j.jocn.2012.06.008. [Epub ahead of print] PMID: 23453155

15

Ratchatawipasanan C, Sirivattanauksorn V, Srisawat C, Chuthapisith S. Roles of circulating tumour cells in breast cancer. J Assoc Gen Surg Thailand 2012;8(17):44-48

16

Hatairaktham S, Srisawat C, Siritanaratkul N, Chiangjong W, Fucharoen S, Thongboonkerd V, Kalpravidh RW. Differential plasma proteome profiles of mild versus severe β-thalassemia/Hb E. Ann Hematol. 2012 Nov 17.

17

Pongtepaditep S, Limjindaporn T, Lertrit P, Srisawat C, Limwongse C. Polyglutamined expanded androgen receptor interacts with chaperonin CCT. Eur J Med Genet. 2012 Jul 14.

18

Sirivatanauksorn Y, Sirivatanauksorn V, Srisawat C, Khongmanee A, Tongkham C. Differential expression of sprouty genes in hepatocellular carcinoma. J Surg Oncol 2012;105(3):273-6.

19

Jungtrakoon P, Plengvidhya N, Tangjittipokin W, Chimnaronk S, Salaemae W, Chongjaroen N, Chanprasert K, Sujjitjoon J, Srisawat C, Yenchitsomanus PT. Novel adiponectin variants identified in type 2 diabetic patients reveal multimerization and secretion defects. PLoS One 2011;6(10):e26792.

20

Nagila A, Netsawang J, Srisawat C, Noisakran S, Morchang A, Yasamut U, et al. Role of CD137 signaling in dengue virus-mediated apoptosis. Biochem Biophys Res Commun 2011;410(3):428-33.

21

Morchang A, Yasamut U, Netsawang J, Noisakran S, Wongwiwat W, Songprakhon P, Srisawat C, et al. Cell death gene expression profile: Role of RIPK2 in dengue virus-mediated apoptosis. Virus Res 2011;156(1-2):25-34.

22

Panya A, Sawasdee N, Srisawat C, Yenchitsomanus PT, Peerapittayamongkol C. Expression of zinc finger and homeobox 2 in erythroleukaemic cells and gamma-globin expression. ScienceAsia 2010;36:342-5.

23

Kirawanich P, Pausawasdi N, Srisawat C, Yakura SJ, Islam NE. An FDTD Interaction Scheme of a High-Intensity Nanosecond-Pulsed Electric-Field System for In Vitro Cell Apoptosis Applications. IEEE Transactions on Plasma Science 2010;38 (10 PART 1), art. no. 5404942, pp. 2574-82.

24

Srisawat C, Engelke DR. Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: strand invaders. Nucleic Acids Res 2010;38(22):8306-15.

25

Phasukkijwatana N, Kunhapan B, Stankovich J, Chuenkongkaew WL, Thomson R, Thornton T, Bahlo M, Mushiroda T, Nakamura Y, Mahasirimongkol S, Tun AW, Srisawat C, et al. Genome-wide linkage scan and association study of PARL to the expression of LHON families in Thailand. Hum Genet 2010;128(1):39-49.

26

Moongkarndi P, Srisawat C, Saetun P, Jantaravinid J, Peerapittayamongkol C, Soi-Ampornkul R, et al. Protective Effect of Mangosteen Extract against beta-Amyloid-Induced Cytotoxicity, Oxidative Stress and Altered Proteome in SK-N-SH Cells. J Proteome Res 2010;9(5):2076-86.

27

Limjindaporn T, Wongwiwat W, Noisakran S, Srisawat C, Netsawang J, Puttikhunt C, et al. Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production. Biochem Biophys Res Commun 2009;379(2):196-200.

28

Walker SC, Scott FH, Srisawat C, Engelke DR. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes. Methods Mol Biol 2008;488:23-40.

29

Chularojanamontri L, Tuchinda C, Srisawat C, Neungton N, Junnu S, Kanyok S. Utility of plasma fluorometric emission scanning for diagnosis of the first 2 cases reports of variegate porphyria: a very rare type of porphyrias in Thai. J Med Assoc Thai 2008;91(12):1915-9.

30

Peerapittayamongkol C, Srisawat C, Junnu S, Kanyok S, Soiampornkul R, Poonsawas I, et al. Porphyrias diagnosed types by HPLC analysis: 10 years Siriraj experience. Siriraj Med J 2007;59:356-60.

31

Tharaphan P, Chuenkongkaew WL, Luangtrakool K, Sanpachudayan T, Suktitipat B, Suphavilai R, Srisawat C, et al. Mitochondrial DNA haplogroup distribution in pedigrees of Southeast Asian G11778A Leber hereditary optic neuropathy. J Neuroophthalmol 2006;26(4):264-7.

32

Suwantarat N, Srisawat C, Masaratana P, Soiampornkul R, Junnu S, Roubsanthisuk W, et al. Microalbuminuria analysis in Thai patients with diabetes and hypertension using albumin blue 580 fluorescence assay. Siriraj Med J 2006;58:1050-3.

33

Atchaneeyasakul LO, Appukuttan B, Pingsuthiwong S, Yenchitsomanus PT, Trinavarat A, Srisawat C, et al. A novel H572R mutation in the transforming growth factor-beta-induced gene in a Thai family with lattice corneal dystrophy type I. Jpn J Ophthalmol 2006;50(5):403-8.

34

Xiao S, Day-Storms JJ, Srisawat C, Fierke CA, Engelke DR. Characterization of conserved sequence elements in eukaryotic RNase P RNA reveals roles in holoenzyme assembly and tRNA processing. Rna 2005;11(6):885-96.

35

Junnu S, Srisawat C, Jantarapassvorn S, Soiampornkul R, Wattanaraksakul N, Neungton N. Comparative study of two screening tests for urinary porphyrins in the diagnosis of porphyrias. Siriraj Med J 2005;57:531-6.

36

Srisawat C, Sanpakit K, Wisutsareevong W, Leenutaphong W, Permpikul C, Neungton N. A girl with a glowing tooth: a case of congenital erythropoietic porphyria. Siriraj Hosp Gaz 2004;56:26-32.

37

Srisawat C, Houser-Scott F, Bertrand E, Xiao S, Singer RH, Engelke DR. An active precursor in assembly of yeast nuclear ribonuclease P. Rna 2002;8(10):1348-60.

38

Srisawat C, Engelke DR. RNA affinity tags for purification of RNAs and ribonucleoprotein complexes. Methods 2002;26(2):156-61.

39

Srisawat C, Goldstein IJ, Engelke DR. Sephadex-binding RNA ligands: rapid affinity purification of RNA from complex RNA mixtures. Nucleic Acids Res 2001;29(2):E4.

40

Srisawat C, Engelke DR. Streptavidin aptamers: affinity tags for the study of RNAs and ribonucleoproteins. Rna 2001;7(4):632-41.

41

Jiang P, Atkinson MR, Srisawat C, Sun Q, Ninfa AJ. Functional dissection of the dimerization and enzymatic activities of Escherichia coli nitrogen regulator II and their regulation by the PII protein. Biochemistry 2000;39(44):13433-49.

42

Wasant P, Liammongkolkul S, Srisawat C. Neonatal screening for congenital hypothyroidism and phenylketonuria at Siriraj Hospital, Mahidol University, Bangkok, Thailand--a pilot study. Southeast Asian J Trop Med Public Health 1999;30 Suppl 2:33-7.

43

Srisawat C, Neungton N, Soiampornkul R. Establishment of OVS3 monoclonal antibody recognizing human ovarian cancer. Siriraj Hosp Gaz 1995;47:909-15.

44

Neungton N, Moongkarndi P, Neungton S, Laohathai K, Srisawat C, Wachirutmanggur L, et al. Establishment of OVS1 and OVS2 monoclonal antibodies recognizing human ovarian mucinous cystadenocarcinoma. Asian Pac J Allergy Immunol 1995;13(1):47-53.

45

Neungton N, Chearskul S, Neungton S, Srisawat C, Angpitakpan D, Sothanayongkul R, et al. Evaluation of new ovarian cancer markers, STN, CA 546 and CA 72-4 in Thai patients. Siriraj Hosp Gaz 1993;45:307-14.

 

 

Abstracts/presentations

 

1

Chotipanang K, Junnu S, Phuangphan P, Densupsoontorn N, Srisawat C. A microassay method for erythrocyte transketolase activity.การประชุมวิชาการโภชนาการแห่งชาติ ครั้งที่ 10 The 10th Thailand Congress of Nutrition (10th TCN 2016) 18 - 20 ตุลาคม 2559  ณ ศูนย์นิทรรศการและการประชุมไบเทค บางนา กรุงเทพฯ

2

Veerataveeporn N, Srisomsap C,Chokchaichamnankit, Srisawat C, Svasti J, Kalpravidh RW.  Proteomic profile of coagulation proteins in β-thalassemia/Hb E patients treated with curcumioids antoxdant cocktail. The 26th Annual Meeting of The Thai Society for Biotechnology and International Conference, Mea Fah Luang University Chiang Rai , Thailand 26-29 November 2014

3

Thazin K, Srisawat C. GENERATION OF NUCLEASE-RESISTANT RNA APTAMERS AGAINST HEMOGLOBIN E. International Conference on Structural and Functional Genomics (ICSAFG) School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India 6th & 7th January 2013

4

Siriboonpipattana S, Srisawat C. Human Serum Albumin Aptamers: A Potential Albumin Removal Tool for Plasma Proteome Analysis. International Conference on Structural and Functional Genomics (ICSAFG) School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India 6th & 7th January 2013

5

Moongkarndi P, Srisawat C, Saetun P, Jantaravinid J, Peerapittayamonkol C, Soi-ampornkul R, Junnu S, Sinchaikul S, Chen ST, Charoensilp P, Thongboonkerd V, Neungton N. Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. มหกรรมสมุนไพรแห่งชาติครั้งที่ 8, 31 สิงหาคม – 4 กันยายน 2554, อิมแพค เมืองทองธานี นนทบุรี.

6

Moongkarndi P, Srisawat C, Saetun P, Jantaravinid J, Peerapittayamonkol C, Soi-ampornkul R, et al. Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. Joint Conference in Medical Sciences 2011: Chula-Rama-Siriraj (JCMS2011), June 15-17, 2011 at IMPACT Exhibition and Convention Center, Thailand: 18.

7

Morchang A, Yasamut U, Netsawang J, Noisakran S, Wongwiwat W, Songprakhon P, Srisawat C, et al. Cell death gene expression profile: Role of RIPK2 in dengue virus-mediated apoptosis. Joint Conference in Medical Sciences 2011: Chula-Rama-Siriraj (JCMS2011), June 15-17, 2011 at IMPACT Exhibition and Convention Center, Thailand: 9.

8

Srisawat C, Engelke DR. Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: strand invaders. Joint Conference in Medical Sciences 2011: Chula-Rama-Siriraj (JCMS2011), June 15-17, 2011 at IMPACT Exhibition and Convention Center, Thailand: 68.

9

Kirawanich P, Pausawasdi N, Srisawat C, Wilairat D, Neatpisarnvanit C. Numerical schemes of nanosecond pulsed electric field systems for cell apoptosis applications. ECTI-CON 2010 - The 2010 ECTI International Conference on Electrical Engineering/Electronics, Computer, Telecommunications and Information Technology , art. no. 5491543, pp. 6-10

10

Nakponetong K, Srisawat C, Benjasupattananun P, Suratannon C, Wachairutmanggur L, Boonchoo S, Pankaew D, Laochareonkait A, Pacharn P, Jirapongsananuruk O, Visitsunthorn N, Vichyanond P. Pharmacokinetics of epinephrine absorption via intranasal administration: a preliminary report. AAAI meeting 2010. 24th American academy of allergy, asthma, and immunology (AAAAI) annual meeting. February 26-March 2, 2010 at New orleans, LA, USA.

11

Summathed T, Manaso J, Sirivattanauksorn V, Srisawat C. Identification of the RNA motif capable of interacting with nickel. งานประชุมวิชาการประจำปี 2553 “โรงเรียนแพทย์ศิริราชเพื่อสังคม 120 ปีแห่งความก้าวหน้า” ระหว่างวันที่ 16 – 20 สิงหาคม 2553  ณ คณะแพทยศาสตร์ศิริราชพยาบาล มหาวิทยาลัยมหิดล

12

Panya A, Srisawat C, Sawasdee N, Yenchitsomanus PT, Peerapittayamongkol C. Human zinc finger and homeodomain 2 (ZHX2) and g-globin expression in erythroleukemic cell line. The 1st CMU Graduate Research Conference, November 27-28, 2009 at Chiang Mai University, Chiang Mai, Thailand.

13

Tit-oon P, Panyawirunroj T, Chuangsuwanich A, Limwongse C, Srisawat C. Inhibition of collagen production in keloid fibroblasts using RNA interference. The 12th National Graduate Research Conference, February 12-13, 2009 at Khon Kaen University, Khon Kaen, Thailand.

14

Neungton N, Moongkarndi P, Srisawat C, Jantaravinid J, Peerapittayamongkol C, Soiampornkul R, et al. Protective effect of mangosteen extract against beta-amyloid-induced apoptosis in SK-N-SH cells. Alzheimer's&dementia J 2009;5:413-4.

15

Tit-oon P, Panyawirunroj T, Chuangsuwanich A, Limwongse C, Srisawat C. Inhibition of collagen production in keloid fibroblasts using RNA interference. Joint Conference in Medical Sciences. 2009:119.

16

Summathed T, Srisawat C. Development of nickel-binding RNA ligands as an RNA affinity tag for pubification of ribonucleoproteins. Joint Conference in Medical Sciences. 2009:31.

17

Limjindaporn T, Wongwiwat W, Noisakran S, Srisawat C, Netsawang J, Puttikhunt C, et al. Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production. Joint Conference in Medical Sciences. 2009:85.

18

Khongmanee A, Sirivatanauksorn Y, Srisawat C, Tongkham C, Sirivatanauksorn V. Expression of sprouty genes in hepatocellular carcinoma. Joint Conference in Medical Sciences. 2009:32.

19

Jantaravinid J, Srisawat C, Moongkarndi P, Neungton N. Protective effect of mangosteen extract against oxidative stress induced by beta-amyloid in SK-N-SH cells. Proceedings of the 12th National Graduate Research Conference by Dean of Graduate Schools of Public University (DGPU) and Khon Kaen University, February 12-13, 2009 at Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand.

20

Jantaravinid J, Srisawat C, Moongkarndi P, Neungton N. Protective effect of mangosteen extract against oxidative stress induced by beta-amyloid in SK-N-SH cells. Joint Conference in Medical Sciences. 2009:118.

21

Tirawanchai N, Chanarat N, Somkasetrin A, Chanchairujira T, Srisawat C, Peerapittayamongkol C, et al. Study on the association of C677T and A1298C polymorphisms of the gene encoding methylenetetrahydrofolate reductase (MTHFR) enzyme and plasma homocysteine level in That hemodialysis patients. 33rd FEBS Congree & 11th IUBMB Conference, June 28-July 3, 2008 at Athens, Greece.